The 30-year-old Bolt, beaten into third in the individual 100m behind Gatlin and his U.S. team compatriot Christian Coleman, was comforted by teammates after getting to his feet, his face etched in pain.
The 4x100m relay witnessed the disaster, as Bolt crumpled on the race track clutching his leg in visible pain and tears, reports CNN.
Bolt received the baton with Jamaica in third, but halfway down the finishing straight the towering sprinter pulled up clutching his left thigh, eventually doing a forward somersault to the ground, to gasps from the 60,000 sell out London Stadium.
Mitchell-Blake, who ran the final leg, said: "I wasn't sure if I had won or not, I gave it my all but I could see Christian Coleman out of the corner of my eye".
Bolt, having been passed the baton by Yohan Blake, was in third behind Great Britain and the United States, who eventually won gold and silver, but pulled up nearly 50 metres from the line and fell to the track. It's a massive team effort and we win as a team and lose as a team. I understand the magic of TV and that everything has to happen for viewers at home but we were held a little long before we went out.
Jamaican team doctor Dr Kevin Jones later confirmed that Bolt had suffered from "cramp in his left hamstring".
"It was ridiculous. We were there waiting, and we were really trying to stay warm, but it was really ridiculous, we waited a really long time", McLeod said, via Jamaica-Gleaner. He's a performer and I think we can all appreciate it. "Usain Bolt's name will always live on".
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"It was insane", added the 2011 world 100m champion Blake.
"Usain was getting cold and he said "I don't like this".
Blake said: "I'm very distraught".
The USA took silver, while Japan grabbed the bronze.
There will be a fair amount of emotion from the stands as Mo Farah also runs in his last track race - the 5,000 metres final.
Team-mate Julian Forte, running in his first World Championships, added: "I really wanted to be a part of the team that sends Usain off in style". "We are on top of the world".